mesenchymal stem cells journals
Madindoline A Affects the Osteogenic Potential and the Wnt Signaling Pathways during Osteogenic Differentiation of Human Mesenchymal Stem Cells in vitro
Mesenchymal stem cells (MSCs) differentiate to mature osteoblasts during a process named osteogenesis, which is characterized by upregulated alkaline phosphatase (ALP) activity and formation of a calcium-rich mineralized extracellular matrix (ECM).
Several signaling pathways are involved in regulation of osteogenesis and direct MSCs into the osteogenic lineage, where the runt-related transcription factor 2 (RUNX2) is the master switch for osteogenesis . Also the Wnt signaling pathways are essential for the guidance of MSCs towards the osteoblastic lineage. The canonical Wnt signaling pathway is implicated as the dominant mechanism in bone biology. Read More>>>>>
Bone marrow-derived mesenchymal stem cells have the capacity to differentiate into several cell types. Fibroblasts are referred to as mature counterpart of MSCs , even though they can differentiate into follicular dendritic cells and myofibroblasts , the latter type of cells play an important role in wound healing. In addition, fibroblasts contribute to synthesis and remodeling of extracellular matrix (ECM) proteins and are able to respond to external stimuli by production of several cytokines, e.g. CCL2, IL6 , and IL 8. Fibroblast-derived cytokines have an impact on the immune system in Graves’ disease and tumor tissue.
In this study we show that the fibroblasts exhibit a similar surface protein expression profile when compared to MSC. Furthermore, we demonstrate expression of 4-prolylhydroxylase and fibronectin in both cell types. Microarray analysis of gene expression also revealed many similarities between MSCs and fibroblasts. These data suggest that MSCs and tissue-resident fibroblasts are closely related, if not even representing two variants of the same cell.
Materials and Methods: Isolation and cell culture of MSCs and fibroblasts: Bone marrow samples were collected from healthy donors after informed consent. MSCs were isolated via their plastic adherence. Briefly, mononuclear cells were isolated from bone marrow by a Ficoll-Paque Plus (Amersham Biosciences AB, Uppsala, Sweden). After one day the non-adherent cells were removed by replacing the medium.Fibroblasts were generated according to a protocol by Rodemann and Bayreuther (1984). Read more………………..